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CDNA Synthesis Kit

Order Now For Only $79. The Most Detailed & Accurate DNA Ancestry Test Of 2020 Western Blot Expression-Verified. Start @ $98 and Next-Day Shipmen Description The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time. Key Features and Benefit

cDNA Synthesis Kits Reliance Select cDNA Synthesis Kit The Reliance Select Kit enables researchers to synthesize cDNA with the highest level of sensitivity and robustness by utilizing the unique properties of its novel chimeric reverse transcriptase. Detection of very rare transcript cDNA synthesis kits We offer a wide range of kits for cDNA synthesis. Use the menu below to select products by application OR sample type cDNA synthesis kits—SMART technology SMART (S witching M echanism a t 5' End of R NA T emplate) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first-strand synthesis, without adapter ligation Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42-50°C and is suitable for synthesis of cDNA up to 13 kb

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The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. qScript reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives Optimized for use with the EVeryRNA EV Purification System, the EVeryRNA cDNA Synthesis & Pre-amplification Kit provides high quality exosomal cDNA for your downstream applications such as PCR, qPCR, miRNA profiling, and RNA-seq

microScript microRNA cDNA Synthesis Kit (Cat. 54410, 54415 When performing reverse transcription qPCR and cDNA synthesis for qPCR, you need reagents that deliver high specificity and sensitivity and process safety. Our RT-qPCR kits have a unique combination of in-process controls - including a built-in visual indicator - to remove variables and increase. The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR

New England BioLabs® - LunaScript® RT SuperMix Ki

The SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies kit, and most can easily be dissolved back into solution by gently mixing the bulk first-strand reaction mix prior to use. First-strand cDNA synthesis Depending on the intended use of the first-strand cDNA, synthesis may be performed using different volumes of bulk first-strand cDNA mix, and different types and amounts of primer and RNA

Your kit will come with step-by-step instructions about how to collect and send us a

cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-5 μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT) 18 and random hexamer primers Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives. Linear target amplification for real-time PCR; Higher yields and precision than other cDNA synthesis kits at a fraction of the cos The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two-step RT-PCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. The optimized blend of random and oligo(dT) primer

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The kit provides all reagents required for first-strand cDNA synthesis reactions. For priming, three different primer systems can be used. Two cDNA synthesis primers are provided with the kit: random hexamer primers and an anchored-oligo(dT) 18 primer. The latter is designed to bind at the beginning of the poly(A) tail to generate full-length. First-strand cDNA Synthesis Kit. 1 Product Result | Match Criteria: Product Name, Property, Description GE27-9261-01 ; Cytiva, 27-9261-01; pricing. SDS; Transcriptor High Fidelity cDNA Synthesis Kit. 1 Product Result. SCRIPT cDNA Synthesis Kit contains all reagents required for first strand cDNA synthesis in one box combining simple handling with high flexibility. The premium quality Reverse Transcriptase, ultrapure dNTPs and an optimized reaction buffer ensure superior results with highest reproducibility. The kit is optimized for high efficiency in a broad. qPCRBIO cDNA Synthesis Kit High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR

The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability Description: The FastSCRIPT cDNA synthesis kit contains everything to generate unbiased, efficient, sensitive high-quality cDNA for downstream experiments ,10002-280EA,10002-278EA AffinityScript Multiple Temperature cDNA Synthesis Kit , Agilent Technologie The RNA-Quant cDNA Synthesis Kit prepares any RNA for qPCR. The poly-A tailing and tagging method in the RNA-Quant System generates cDNA that is highly unique and, when used with the 3' Universal Reverse Primer, does not produce any signal in the absence of reverse transcriptase or lead to the detection of any residual genomic DNA EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the.

The Reliance Select cDNA Synthesis Kit is the newest reverse transcription product from Bio-Rad. This high-performance kit features Reliance Reverse Transcriptase, a novel chimeric enzyme that delivers superior results across a broad dynamic range, even with challenging samples The kit contains all necessary components including a highly sensitive and thermostable recombinant reverse transcriptase which provides enhanced cDNA synthesis efficiency, high fidelity, and a wide range of activity for varying amounts of RNA templates (0.1 ng to 2 µg) The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends. The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor priming methods for cDNA synthesis. - RT Enhancer is included to remove contaminating DNA, eliminating the need for DNAse I treatment. Kit Contents Vial Pack Size (cap color) A B Verso Enzyme Mix 40µl (black) 100µl (black) 5X cDNA synthesis Buffer 500µl (red) 500µl (red) Anchored Oligo-dT (500 ng/µl) 40µl (orange) 100µl (orange

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The µMACS and MultiMACS cDNA Synthesis Kits facilitate fast and efficient mRNA isolation, cDNA Synthesis, and cDNA purification, all in one column, without prior preparation of total RNA. Pure mRNA is directly isolated from cells, tissue, or blood by magnetic labeling with µMACS Oligo(dT) MicroBeads. Due to the fast reaction kinetics the small (50 nm) µMACS Oligo(dT) MicroBeads instantly. Hi, in our lab we study gene expression in plants and good RNA and cDNA synthesis is very important. we used to use SuperScript™ but now we are using iScript cDNA synthesis kit from Bio-rad. it. cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription. Reverse transcriptases (RTs) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR) cDNA synthesis kits provide a fast and reliable means of producing cDNA from RNA samples. These convenient ready-to-use kits come with the components necessary for reverse transcription, including the reverse transcriptase (RT), dNTPs, reaction buffer, nuclease-free water, and RNase inhibitors Our qScript Flex cDNA Synthesis kit allows the flexibility to choose the method of your choice (oligo-dT, random primer, gene-specific primer, or any combination thereof) for priming first-strand synthesis. We have been able to generate cDNAs that were over 15-kb long with this kit. How the denaturation step is carried out is very important

The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation. The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT) 18 and random hexamer primers qScript™ cDNA Synthesis Kit is a sensitive and easy to use kit for RNA quantification using two-step RT-PCR. This kit contains an optimised blend of random and oligo (dT) primers for robust, consistent and unbiased first-strand synthesis over a broad range of RNA template concentrations iScript cDNA Synthesis Kit produces excellent results in both real-time and conventional RT-PCR. Storage and Stability Store the iScript™ cDNA Synthesis Kit at -20 °C in a constant temperature freezer. When stored under these conditions the kit components are stable for a minimum of one year after ship date The ProtoScript First Strand cDNA Synthesis Kit is contains everything needed for cDNA synthesis. Recombinant M-MuLV reverse transcriptase Generates cDNA up to 10 kb Supplied with Oligo-dT primers, Randomized Primer Mix, and nuclease-free wate The kit uses M-MLV reverse transcriptase with lower RNase H activity, which avoids degradation of RNA when RNA-DNA hybrid forms in the progress of cDNA synthesis. It is suitable for synthesis of cDNA up to 9 kb. The cDNA products could be used directly in a variety of downstream applications, including PCR, qPCR, gene clone. Cautio

microScript microRNA cDNA Synthesis Kit (Cat

I have purified RNA and synthesized cDNA using QuantiTech Reverse Transcriptase Kit from Qiazen. I want to to do PCR for a specific gene of interest. Is it possible to check the quality of cDNA The First-Strand cDNA Synthesis Kit contains all the components needed to synthesize the first-strand cDNA and provides two cDNA synthesis primers, Random Primers and Oligo (dT)23VN. You can choose Random Primers, Oligo (dT)23VN or gene-specific primers as reverse transcription primers according to the needs of the experiment

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The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are. qPCRBIO cDNA Synthesis Kit can transcribe as little as 4pg and up to 0.4μg of RNA. The performance depends on the type of RNA, RNA quality and whether specific primers, oligod(Ts) or random hexamers are used. For higher capacity applications please use UltraScript 2.0 Reverse Transcriptase and cDNA Synthesis Kits Protocol for cDNA synthesis and qRT-PCR cDNA Synthesis Superscript III 1st Strand Synthesis Kit (Invitrogen, Cat #18080-051) We typically use 1μg of Total RNA per 20μl cDNA reaction, but you may be able to use less. Initial Primer/RNA Mix: 0.5 μl random hexamer primers 0.5 μl oligo(dT) primers 1μl dNTP mix 8ul of RNA (1μg) + DEPC H 2

iScript™ cDNA Synthesis Kit Life Science Research Bio-Ra

RevertAid First Strand cDNA Synthesis Kit

cDNA Synthesis Kits Life Science Research Bio-Ra

  1. reaction tim
  2. The 2-tube UltraScript 2.0 cDNA Synthesis Kit contains all the components required for fast, reliable and unbiased cDNA synthesis, including an optimised blend of anchored oligo(dT) and random hexamers. UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation.
  3. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies.A novel, highly-pure reverse transcriptase and new TransAmp™ buffer system delivers highly.
  4. Accuris now offers two different cDNA synthesis kits, to meet a range of requirements. Our original cDNA Synthesis Kit comes as a convenient 2-tube format for easy reaction set up and is ideal for 4pg to 0.5µg of input RNA.. The new First Strand cDNA Synthesis Flex Kit includes four components: Reverse Transcriptase, Buffer, oligo (dT) primers, and random hexamer primers
  5. The OrientExpress™ cDNA Synthesis and Cloning Systems are complete sets of reagents designed for rapid, efficient construction of cDNA libraries having inserts in a defined orientation. Six systems are available based on the following vectors: a bacteriophage lambda insertion vector, λSCREEN-1, and two bacteriophage T7 vectors, T7Select1-1.
  6. A protocol for a kit that includes a reverse transcriptase and reagents for synthesis of first-strand cDNA optimized in preparation for PCR amplification. The kit can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA
  7. g covering the entire RNA.

cDNA synthesis kits - Takara Bi

Home / Product- Molecular Reagents / RT-PCR/Real Time PCR / MG cDNA Synthesis Kit II MG cDNA Synthesis Kit II mgmed 2017-08-21T15:00:43+00:00 Product- Molecular Reagent SuperScript® Double-Stranded cDNA Synthesis Kit Catalog no. 11917-010 Size: 10 reactions Catalog no. 11917-020 Size: 50 reactions Store at -20°C Description The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents—except an oligo(dT) containing primer— necessary to make double-stranded cDNA from total RNA or poly A + selected RNA (mRNA)

SMARTer cDNA synthesis kits - Takara Bi

The Right Solution for miRNA Quantification | Biocompare

RevertAid First Strand cDNA Synthesis Ki

The miRNA cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand miRNA from total RNA templates. The kit utilizes a mutated recombinant M-MuLV Reverse Transcriptase which exhibits limited RNase H activity. The recombinant RNasin™ Ribonuclease Inhibitor, supplied with the kit effectively protects the RNA template. Choose your priming methods with abm?s cDNA Synthesis Kit. abm?s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers

The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time cDNA synthesis primers are provided with the kit: random hexamer primers and an anchored-oligo(dT)18 primer. The latter is designed to bind at the beginning of the poly(A) tail in order to generate full-length cDNAs and to prevent prim-ing from internal sites of the poly(A) tail. The 5´-ends of lon STEMscript™ cDNA Synthesis Kit includes three components that have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification: STEMscript™ Reverse Transcriptase Mix (contains STEMscript™ Reverse Transcriptase purified from recombinant E. coli, and recombinant ribonuclease inhibitor), STEMscript™ Reaction Buffer (contains primers, MgCl₂. The BioLink™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high-quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. It is ideal for 4pg to 0.5µg of input RNA and includes two tubes for easy reaction set up

The FastSCRIPT™ cDNA Synthesis Kit contains everything you need to generate unbiased, efficient, sensitive and high-quality cDNA for your downstream experiments. Our kit removes the need for user optimization, making it an ideal solution due to its robust performance and ease of use The Radiant™ cDNA Synthesis Kit uses the latest developments in reverse transcriptase technology and buffer chemistry to enhance cDNA synthesis speed and yield with accurate transcript representation. The reverse transcriptase, buffer system and combination of random hexamers with anchored oligo(dT) allow for unbiased, efficient, sensitive. Abcam's Hi-Fi cDNA Synthesis Kit (ab185916) is optimized to synthesize a DNA copy specifically using bisulfite-converted RNA and enriched RNA fragments. The kit is also suitable for cDNA synthesis using total RNA. The synthesized DNA can then be used for a variety of downstream applications including PCR and cDNA library construction..

Uses: The EpiNext™ Hi-Fi cDNA Synthesis Kit is optimized to synthesize a DNA copy specifically using bisulfite-converted RNA and enriched RNA fragments. The kit is also suitable for cDNA synthesis using total RNA. The synthesized DNA can then be used for a variety of downstream applications including PCR and cDNA library construction The Direct cDNA Sequencing Kit is used to prepare cDNA for nanopore sequencing without using PCR. The kit recommends an input of 100 ng poly-A + RNA. Taking full-length poly-A + RNA, complementary strand synthesis and strand switching are performed using kit-supplied oligonucleotides. The RNA template is then digested, and the second. 3. Prepare the following cDNA Synthesis Mix, add the following components in the indicated order: 5x first-strand buffer 4 µl dNTPs(10 mM each) 1 µl RNasin (40U/μl) 0.5 µl M-MLV 1 µl 4.Mix gently and centrifuge 5.For oligo(dT)18, incubate for 60 min at 42°C. For random hexamer primed synthesis, incubate for 60 min at 37°C

qScript microRNA cDNA Synthesis Kit | PCR and cDNA

qScript cDNA Synthesis Kit First-Strand cDNA Synthesis

The TruScript First Strand cDNA Synthesis Kit is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts). This kit contains the 2x Reaction Mix and the TruScript Enzyme Mix for effective cDNA synthesis from total RNA transcripts M-MLV cDNA Synthesis kit provides a sensitive and easy-to-use solution for two-step RT-PCR. This kit includes five tubes comprehensive of the reagents required for successful RT-PCR. The M-MLV H-reverse transcriptase is optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The enzyme is exceptionally well with a wide variety of targets MG cDNA Synthesis Kit MG cDNA Synthesis Kit II MG 2X qPCR MasterMix (SYBR Green) MG 2X qPCR MasterMix (TaqMan Download Manual. Viva cDNA Synthesis Kit-50 reaction Viva cDNA Synthesis Kit-100 reaction. Publication This Product Has Been Used In: Ahmad Asnawi MUS et al. (2020) Total RNA extraction from the aromatic Phalaenpsis bellina, endemic orchid in Sabah, Borneo, Walailak Journal of Science & Technology, 18(1):6553. Azad, A.R. et al. (2020) The Prevalence of Hepatitis B and D Viruses and Evaluating. Description: The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility

High-Capacity cDNA Reverse Transcription Ki

GoldBio's First Strand cDNA synthesis kit includes our 5x Master Mix which contains: Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl 2 and protein stabilizers. Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability cDNA synthesis core kit. Filters. One Step kit converter; PROBE One-Step; cDNA synthesis core kit; 1 - 1 of 1. Reverse Transcriptase Core Kit Quick view 1 - 1 of 1. Products ; Endpoint PCR; Quantitative PCR ; Gene purification & Extraction; Electrophoresis System. Application Area: cDNA for qPCR Great kit for the synthesis of cDNA, is very consistent. However, if you are utilizing a small amount of RNA this kit isn't that great. Would recommend a more sensitive RT PRODUCTS | cDNA synthesis kits | ReverTra Ace™ qPCR RT Master Mix with gDNA Remover | The category leader, continuing to create new value that contributes to society in the environment, healthcare, and high-function product fields

EVeryRNA™ cDNA Synthesis & Pre-Amplification Kit System

microScript microRNA cDNA Synthesis Kit (Cat

  1. 2.1 mRNA isolation and cDNA synthesis Warm all buffers of the µMACS cDNA Kit to room temperature. For mRNA isolation from whole blood, we recommend to use PAXgene® Blood RNA Tubes (PreAnalytiX®) for RNA stabilization in combination with the μMACS One-step cDNA Kit (# 130-091-902)
  2. utes. This kit is suitable for the synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries. Storage / Shipping: Ship at 4°C ; Store at -20°C : Shelf Life: Store at -20°C for 12 months.
  3. Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates The kit uses RevertAid Reverse Transcriptase RT a recombinant M MuLV RT which maintains activity at 42 50°C and is suitable for synthesis of cDNA up to 13 kb RiboLock RNase Inhibitor supplied with the kit effectively protects RNA templates from.
  4. First-Strand cDNA Synthesis Kit Introduction The First-Strand cDNA Synthesis Kit is based on Reverse Transcriptase (Cat. No. K1071) to synthesize first-strand cDNA from Total RNA or Poly(A)+ RNA. Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, Reverse.
  5. The BlazeTaq™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification
  6. SuperScript® VILO™ cDNA Synthesis Kit Cat. no: Size: 11754-050 50 reactions (20 µl/reaction) 11754-250 250 reactions (20 µl/reaction) Store at -20°C (non-frost-free) 製品概要 SuperScript® VILO™ cDNA Synthesis Kitは、リアルタイム定量RT-PC
  7. cDNA synthesis. This kit is designed for use with 1 pg-5 μg of total RNA or 1 pg-500 ng of poly(A)+ RNA. For >5 μg total RNA, increase reaction volumes and amount of SuperScript® III RT proportionally. • RNaseOUT™ Recombinant RNase Inhibitor has been added to the system to safeguard against degradation of target RNA due t

Highly specific cDNA synthesis for qPCR - Qiage

  1. RT-PCR success. This kit uses a streamlined protocol, which makes it easy and fast to set up multiple cDNA synthesis reactions, saving you valuable research time. Additionally, using this kit for cDNA synthesis provides you the flexibility to select the PCR enzyme or master mix that is best suited to your downstream application
  2. Figure 2 shows that cDNA prepared with the Marligen's First-strand cDNA Synthesis System provides significantly enhanced sensitivity compared to cDNA prepared with a competitor's kit. In this experiment, cDNAs prepared using each method were amplified in real time with primers specific for the 5' end of the ADAR gene using three different.
  3. cDNA Synthesis Kit Version November 2008 Cat. No. 05 081 955 001 Kit for 50 reactions (plus 10 controls) Cat. No. 05 091 284 001 Kit for 100 reactions Cat. No. 05 081 963 001 Kit for 200 reactions Store the kit at 15 to 25°C
  4. qMAX™ cDNA Synthesis Kit Rapidly generate high quality, qPCR ready cDNA Unbiased, complete sequence representation Thermostable qMax RT with RNase inhibitor Optimized for use across a wide dynamic range Two tube format for easy reaction set up cDNA is a vital research tool for Molecular Biologists. The qMax DNA Synthesis Kit from Accuris provides
  5. Materials and Kits Needed Preparing cDNA from RNA Samples First-Strand Synthesis First strand cDNA synthesis employs the Clontech SMARTer PCR cDNA Synthesis Kit. The CDS Primer IIA is first annealed to the polyA+ tail of transcripts, followed by first-strand synthesis with SMARTScribe™ Reverse Transcriptase
  6. Empirical's RTScript™ cDNA Synthesis Kit is a modified version of M-MLV reverse transcriptase with RNase H activity deactivated and increased thermal stability. In combination with optimized buffers and reagents, Empirical's RT enzyme synthesizes complementary DNA from single-stranded RNA or DNA templates

AffinityScript cDNA Synthesis Kit - Agilen

Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. Brand: Thermo Scientific K1612 277.09 EUR valid until 2021-06-30 Use promo code 14804 to get your promotional price.. ProtoScript® II First Strand cDNA Synthesis Kit: E6560S by New England Biolabs, Inc. at Labscoop.com - Read reviews, citations, datasheets, protocols & more

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ProtoScript® First Strand cDNA Synthesis Kit NE

  1. The original cDNA Synthesis Kit comes as a convenient 2-tube format for easy reaction set up and is best for 4pg to 0.5µg of input RNA. One tube includes the exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers.
  2. The cDNA Synthesis Kit is supplied with a Reaction Setup Allow kit components to thaw and briefly vortex/centrifuge. Keep tubes on ice. RNA/Primer Mix - Combine components in a nuclease-free microtube on ice: RNA Template 10pg to 2.0µg Total RNA 5pg to 0.5µg mRNA Random Hexaprimer or oligo (dT) 2.0µl Use 3µl for >2µg RNA.
  3. Brand: New England Biolabs The ProtoScript® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA. Recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability (active up to 48°C) Generates cDNA up to 10 kb Supplied with Oligo-dT primers, Randomized Primer Mix, and nuclease-free wate
  4. First-Strand cDNA Synthesis Kit Cytiv
DNA & RNA Purification: mRNA and GenElute Direct mRNAGenoSensor Corporation - microRNA Microarrays and ChipsCMV Promoter based pCDH-CMV-MCS-EF1-GFP-T2A-Puro cDNASolis BioDyne - dNTP Mix - (dATP, dCTP, dGTP and dTTP)
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